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Development and advantages of serum‐free, chemically defined nutrient media for culturing of nerve tissue
Author(s) -
Romijn Herms J.
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1988.tb00749.x
Subject(s) - biology , chemically defined medium , acknowledgement , nerve cells , nutrient , microbiology and biotechnology , biochemistry , in vitro , computer science , ecology , computer security
This paper describes in a historical perspective the development of serum‐free nutrient media suitable for long‐term culturing of nerve tissue. Several disadvantages of the use of serum are discussed, coupled with an acknowledgement that it is not always advisable to replace a routinely used serum‐supplemented medium by a chemically defined medium with the expectation of immediate success. Therefore a strategy is given on how to develop a chemically defined medium that is thoroughly tuned to the specific needs of the cell type to be cultured. It is argued that such a medium has several substantial advantages over the use of serum.