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Anionin sites of the basement membrane of rat seminiferous tubules during ontogenesis
Author(s) -
Leheup Bruno P.,
Gelly JeanLouis,
Delongeas JeanLuc,
Grig Georges
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1988.tb00744.x
Subject(s) - lamina densa , biology , basement membrane , fetus , lamina , lamina lucida , anatomy , biophysics , microbiology and biotechnology , ultrastructure , basal lamina , pregnancy , genetics
The anionic sites of the basement membrane of rat seminiferous tubules were demonstrated ultrastructurally in the lamina densa by using cationic polyethyleneimine (PEI). The sites were largely digested out after incubation with heparitinase, indicating a large proportion of heparan sulfates. The anionic sites were present as early as day 16 of gestation on the interstitial side of the lamina densa, and after gestation day 20 they were symmetrically organized on both sides of the lamina densa. The number of sites is not modified postnatally. They appear more irregular in density with advancing age. Experimental conditions as crytorchidism, fetal irradiation, and ligation of the ductuli efferentes lead to unspecific alterations in the distribution of the anionic sites that are parallel to the modifications in the basement membrane.