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Culture of an epithelial cell line (MDCK) with a serum substitute
Author(s) -
Gutiérrez M.C.,
DíazPontones D.M.,
Vancell M.R. López,
Beaty G.
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1988.tb00726.x
Subject(s) - amiloride , biology , sodium , epithelium , trypsin , cell culture , biophysics , fetal bovine serum , microbiology and biotechnology , efflux , cell , biochemistry , chemistry , enzyme , genetics , organic chemistry
This paper reports the suitability of culturing a line of dog kidney epithelial cells, MDCK, in the presence of a serum substitute, Ultroser G. Serial subcultivation with this product was possible for at least 10 passages without any change in cell shape and size, saturation density, dome‐forming ability, transepithelial resistance, and growth curve. Adhesion of newly plated cells to plastic was somewhat lower than in fetal calf serum but the trypsin‐harvesting kinetics were essentially the same. However, the membrane ion transport systems was alterd: cell sodium influx was greatly diminished, suggesting a deep change in the amiloride‐sensitive Na + channels: sodium efflux was highly enhanced (both active and passive).