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Blastema cell proliferation in vitro : Effects of limb amputation on the mitogenic activity of spinal cord extracts
Author(s) -
Boilly Béi,
Albert Philippe
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1988.tb00720.x
Subject(s) - blastema , forelimb , biology , amputation , spinal cord , regeneration (biology) , axolotl , anatomy , mesenchymal stem cell , microbiology and biotechnology , surgery , neuroscience , medicine
Primary cultures of mesenchymal cells of axolotl limb blastemas provide a very sensitive in vitro bioassay for studying nerve dependence of newt regeneration. These cells can be stimulated by crude spinal cord extracts on non‐amputated animals in a dose‐dependent manner up to 60 μg protein/ml of culture medium; at this concentration the mitotic index is increased 4‐fold. Spinal cord extracts of axolotls 14 days after forelimb amputation ( i.e. , late bud stage) are more efficient in stimulating blastema cell proliferation (+50%) than extracts of axolotls 7 days after forelimb amputation ( i.e. , early bud stage) or of axolotls without amputation. In a similar manner, spinal cord extracts of young axolotls 14 days after forelimb amputation, are more stimulatory than older axolotls 14 d after forelimb amputation which regenerate only a very small blastema during the same time. It appears that spinal cord mitogenic activity is enhanced after limb amputation, probably in correlation with blastema cell requirements for limb regeneration.