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Anti‐bromodeoxyuridine monoclonal antibody: An alternative tool for the identification of replicated DNA at the electron microscope level
Author(s) -
Thiry Marc,
Dombrowicz David
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1988.tb00710.x
Subject(s) - immunogold labelling , biology , bromodeoxyuridine , dna , electron microscope , monoclonal antibody , microbiology and biotechnology , chromatin , antibody , biochemistry , genetics , optics , cell growth , physics
A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5‐bromodeoxyuridine (BUdR) in conjunction with 5‐fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl‐embedded sections by immunogold technique using a monoclonal anti‐BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled.