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Lectin‐like components in the macronuclear replication bands of Euplotes eurystomus
Author(s) -
Olins Donald E.,
Olins Ada L.,
Sève AnniePierre,
Bourgeois Claire A.,
Hubert Jean,
Monsigny Michel
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1988.tb00709.x
Subject(s) - biology , lectin , nucleolus , sugar , labelling , dna , microbiology and biotechnology , dna replication , biochemistry , cytoplasm
Upon incubation with fluoresceinylated neoglycoproteins, isolated macronuclei from the ciliated protozoan Euplotes eurystomus display different labelling patterns depending on the nature of the sugar bound to the neoglycoproteins. Specific sugar‐binding components ( i.e. , lectin‐like molecules) are associated with presumed nucleoli and with the macronuclear replication bands. This is the first demonstration that DNA synthesis and sugar‐binding components are co‐localized in an eukaryotic cell.