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Immunohistochemical localization of type IV collagen in mouse tooth germ: an ultrastructural study
Author(s) -
Brisset L.,
LaurentMaquin D.,
Brossard D.,
Cam Y.
Publication year - 1987
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1987.tb00573.x
Subject(s) - lamina densa , lamina lucida , basement membrane , biology , mesenchyme , odontoblast , mesenchymal stem cell , immunostaining , type iv collagen , ultrastructure , microbiology and biotechnology , pathology , anatomy , immunohistochemistry , extracellular matrix , basal lamina , laminin , immunology , pulp (tooth) , medicine
Localization of type IV collagen was analyzed at the ultrastructural level in mouse embryonic molars by using a preembedding technique. Cryostat sections were incubated with type IV collagen antibody and then treated with the peroxidase‐antiperoxidase complex. This antibody was visualized at the epithelio‐mesenchymal interface. Labeling was intense and uniformly distributed throughout the basement membrane. However, it was mainly restricted to the lamina densa. No immunostaining was detectable in the lamina lucida but it was crossed by fine filaments that appeared as projections from the lamina densa to the epithelial cell plasma membrane. At the mesenchymal aspect of the basement membrane, projections of labeled material extended from the lamina densa in the underlying dental mesenchyme. At the presecretory stage of odontoblasts, these projections were in close connection with mesenchymal cell processes.