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Use of monoclonal antibodies to follow the phylogenic distribution of human renal antigens
Author(s) -
Candelier J. J.,
Pendu J.,
Couillin P.,
Boue A.
Publication year - 1987
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1987.tb00572.x
Subject(s) - mesonephros , biology , monoclonal antibody , pronephros , antigen , antibody , epitope , glomerular basement membrane , fibronectin , kidney , immunofluorescence , staining , extracellular matrix , microbiology and biotechnology , immunology , glomerulonephritis , zebrafish , embryonic stem cell , endocrinology , biochemistry , genetics , gene
Nine human differentiation antigens have been defined by monoclonal antibodies (M. Abs) developed from mice immunized with embryonic kidney cells (mesonephros or metanephros of 7 week‐developmental ages). Their spatial and temporal distributions during human kidney organization were previously studied [3]. In this paper we have attempted to follow by immunofluorescence their phylogenic location, from fish to mammals. Six of them recognized the same structures as in humans: proximal convoluted tubules (PCT) (EG9.11, EG19.6, E116.1), glomerular basement membrane (GBM) (EG14.1) and extracellular matrix (EK8.1, EK17.1). However, staining was limited to certain mammals. EK17.1 has been characterized as an anti‐fibronectin. These antibodies revealed the same histological structures in the human mesonephros and metanephros. The three other antibodies revealed epitopes appearing earlier in evolution and whose histological distribution varied according to species. These antibodies stained different structures in the mesonephros and metanephros. Thus, the staining particularities observed during human renal ontogenesis were found again in the phylogenetical study.