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Inclusion bodies in recombinant E. coli producing human calcitonin tetramer, as visualized by immuno‐gold electron microscopy
Author(s) -
Petrov P.,
Jay E.,
Ivanov I.
Publication year - 1987
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1987.tb00562.x
Subject(s) - inclusion bodies , tetramer , recombinant dna , biology , calcitonin , electron microscope , escherichia coli , microbiology and biotechnology , biochemistry , gene , optics , enzyme , physics , endocrinology
Inclusion bodies are described in recombinant E. coli cells harboring plasmid for the expression of a synthetic gene coding for human calcitonin tetramer. The inclusion bodies are visualized by electron microscopy and the protein is identified by immuno‐gold technique, using antibodies against synthetic human calcitonin. The diameter of the inclusion bodies is 1 micron on the average.