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Ultrastructural localization of actin in the intermediate lobe of rat hypophysis
Author(s) -
Kondo H.
Publication year - 1987
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1987.tb00546.x
Subject(s) - immunoelectron microscopy , biology , actin , ultrastructure , cytoplasm , microbiology and biotechnology , electron microscope , immunofluorescence , colocalization , immunogold labelling , anatomy , immunohistochemistry , antibody , immunology , physics , optics
The localization of actin in the cells of the pars intermedia of rat hypophysis was studied by means of the polyethylene glycol (PEG) embedding and subsequent de‐embedding method together with FITC and IgG‐colloidal gold immunolabelling techniques. Actin immunofluorescence was detected to be punctate throughout the entire cytoplasm, except for the nuclear region. In electron microscopy actin gold‐labelling was localized on portions of microtrabeculae in close association with the secretory granules, but not within the secretory granules themselves. This close association of actin with the secretory granules strongly suggests the involvement of the contractile protein in the cellular secretory process. Several restrictions due to the PEG‐method itself are also discussed to interpret clearly immunoelectron microscope images from the embedment‐free sections.