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Monoclonal antibody against a nuclear matrix antigen in proliferating human cells
Author(s) -
Philipova R. N.,
Zhelev N. Z.,
Todorov I. T.,
Hadjiolov A. A.
Publication year - 1987
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1987.tb00539.x
Subject(s) - nuclear matrix , biology , monoclonal antibody , nucleoplasm , immunoprecipitation , microbiology and biotechnology , antigen , nuclear protein , cell nucleus , antibody , cell culture , proliferating cell nuclear antigen , splenocyte , dna , nucleolus , biochemistry , cytoplasm , immunology , genetics , gene , transcription factor , chromatin
A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human cell lines and phytohemagglutinin‐stimulated lymphocytes, but absence in resting lymphocytes; (b) appearance in stimulated lymphocytes in parallel with the onset of DNA synthesis; (c) a speckled distribution in the nucleoplasm; (d) tight association with nuclear matrix structures identified by both biochemical and in situ extraction and enzyme treatment procedures; (e) mol wt of 125 kDa and pI 6.5 as determined by immunoprecipitation or immunoblotting of nuclear or nuclear matrix proteins fractionated by gel electrophoresis. The above characteristics identify the p125/6.5 nuclear matrix protein recognized by the isolated monoclonal antibody as belonging to the class of proliferating cell nuclear antigens.