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Modulating effects of drugs on cell cycle kinetics
Author(s) -
Ronot X.,
Adolphe M.
Publication year - 1986
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1986.tb00498.x
Subject(s) - cell cycle , biology , cytokinesis , flow cytometry , microbiology and biotechnology , cell , drug , dna , dna synthesis , distribution (mathematics) , rna , cell division , computational biology , pharmacology , genetics , gene , mathematical analysis , mathematics
The effects of individual drugs on cell cycle progression can often be determined by analyzing the DNA distribution of cultured cells at appropriate times after drug administration. In addition, to cell counts, RNA and/or protein content, the alterations in cell cycle distribution of drug‐treated cells can yield information on the potential cell cycle phase specificity of the drug. However single parameter DNA analysis, as currently used, can give inaccurate information when drug effects are associated with cytokinesis perturbations, in spite of various statistical and mathematical analyses. Scanning flow cytometry could be an interesting alternative for the detection of binucleate cells.