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Fibronectin (FN) localizations in early wound healing of cultured frog skins
Author(s) -
Zhu Q. L.,
Denefle J. P.,
Lechaire J. P.
Publication year - 1986
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1986.tb00473.x
Subject(s) - fibronectin , wound healing , cycloheximide , biology , epidermis (zoology) , pathology , anatomy , microbiology and biotechnology , immunology , extracellular matrix , medicine , protein biosynthesis
The wound healing process of frog skin fragments in epibolic cultures has provided information on FN localizations during the migration of keratinocytes. Mainly two FN localizations were studied by indirect immunodetections: Epidermal localization around keratinocytes which have acquired a fibroblastic shape. Dermal localizations of the sectioned collagen of the stratum spongiosum and stratum compactum detected at the beginning of the culture. Both localizations were observed in this epibolic wound healing process during 6 hr and 24 hr in culture and showed a differential sensitivity to cycloheximide (CHX). It was worth noting that fibronectin was permanently detected in the subcutaneous tissue of non‐cultured or cultured skin fragments with or without CHX.