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Identification of two macrophage populations by flow cytometry monitoring of oxidative burst and phagocytic functions
Author(s) -
Lepoivre M.,
Roche A. C.,
Tenu J. P.,
Petit J. F.,
Nolibe D.,
Monsigny M.
Publication year - 1986
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1986.tb00470.x
Subject(s) - flow cytometry , biology , population , macrophage , phagocyte , fluorescence , respiratory burst , microbiology and biotechnology , phagocytosis , liposome , biophysics , immunology , biochemistry , in vitro , physics , demography , quantum mechanics , sociology
Two populations of phagocytic cells from trehalose dimycolate‐elicited mouse peritoneal cells are demonstrated by flow cytofluorometry, using two fluorescent probes excited at the same wavelength (488 nm). Liposomes containing diethylenetriaminepentaacetate daunorubicin conjugate (maximum emission wavelength: 590 nm) allow the discrimination of phagocytes and non‐phagocytic cells. Among the phagocytes, an activated population is revealed by a cell‐associated fluorescence of the oxidation product of dichlorofluorescein diacetate (maximum emission wavelength: 520 nm).