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Primary rat cardiac cell culture: diet of the mother rats as a determinant parameter of cardiomyoblast production from neonates
Author(s) -
Grynberg A.,
Degois M.,
Guenot L.,
Athias P.
Publication year - 1986
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1986.tb00466.x
Subject(s) - biology , pregnancy , andrology , primary culture , fetus , medicine , endocrinology , strain (injury) , zoology , cell culture , physiology , anatomy , genetics
Two populations of Wistar rats from the same strain were fed two commonly used commercial chows. The yields of myocardial cells for primary culture obtained from neonatal rats in these 2 populations were significantly different. This effect is strickly caused by diet used during pregnancy and is quickly reversible from one pregnancy to the following one in the same females. This diet effect is limited to the yield in cardiomyoblasts and cultures were not found different after 5 days in the same medium.