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Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry
Author(s) -
Ronot X.,
Benel L.,
Adolphe M.,
Mounolou J. C.
Publication year - 1986
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1986.tb00458.x
Subject(s) - rhodamine 123 , flow cytometry , biology , organelle , rhodamine , mitochondrion , cytometry , microbiology and biotechnology , fluorescence microscope , fluorescence , biophysics , biochemistry , antibiotics , physics , quantum mechanics , multiple drug resistance
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of the whole cell and its organelles. Rhodamine 123 is a vital fluorescent dye used in flow cytometry. As it is specifically concentrated in mitochondria because of the transmembrane potential that these organelles maintain in living cells, rhodamine 123 is thus a useful probe for monitoring the abundance and activity of mitochondria. A critical survey of the routine use of rhodamine 123 together with flow cytometry in mitochondrial research is presented.