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Inhibitory effect of sodium arsenite and azide on asialoglycoprotein receptor mediated endocytosis in suspended rat hepatocytes
Author(s) -
Scarmato P.,
Durand G.,
Agneray J.,
Feger J.
Publication year - 1986
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1986.tb00457.x
Subject(s) - endocytosis , sodium arsenite , sodium azide , asialoglycoprotein receptor , biology , internalization , receptor mediated endocytosis , receptor , ligand (biochemistry) , microbiology and biotechnology , arsenite , biochemistry , hepatocyte , biophysics , chemistry , in vitro , arsenic , organic chemistry
The inhibitory effect of sodium arsenite and azide on asialoorosomucoid endocytosis was tested using isolated rat hepatocytes. Under either continuous flux conditions or a single synchronous wave of ligand endocytosis we confirm that azide inhibits the recycling of the receptors and we provide evidence for the involvement of thiol groups in the internalization step. In addition pretreatment of hepatocytes with azide allows us to demonstrate that receptor endocytosis proceeds independently of the presence of any specific ligand.