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Dome formation in the human colon carcinoma cell line Caco‐2 in culture. Influence of ouabain and permeable supports
Author(s) -
Ramond M. J.,
MartinotPeignoux M.,
Erlinger S.
Publication year - 1985
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1985.tb00383.x
Subject(s) - ouabain , caco 2 , biology , dome (geology) , cell culture , monolayer , biophysics , cell , atpase , incubation , microbiology and biotechnology , biochemistry , chemistry , sodium , enzyme , paleontology , genetics , organic chemistry
We studied formation of domes in cell monolayers of the human colon carcinoma cell line Caco‐2 which has been shown to exhibit signs of enterocytic differentiation and transport properties. After a 24 hr incubation with 4 × 10(‐8) M ouabain, the number of domes seen on Caco‐2 cell monolayers grown on plastic dishes was not significantly altered. After a 90 min preincubation with ouabain, 86rubidium uptake by Caco‐2 cells was inhibited by ouabain, indicating that the cells have an ouabain‐sensitive Na+, K+‐ATPase, while dome formation was unaffected by ouabain. Domes were observed in Caco‐2 cell monolayers grown on Nuclepore filters when the pore size was 0.015 micron but not when it was 0.030 micron. Our results suggest that dome formation in the Caco‐2 cell line could be independent of Na+, K+‐ATPase activity and might be due to accumulation of molecules having an effective hydrodynamic radius comprised between 0.015 and 0.030 micron.