Premium
Re‐evaluation of the site of transcription in Ehrlich tumour cell nucleoli
Author(s) -
Thiry M.,
Lepoint A.,
Goessens G.
Publication year - 1985
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1985.tb00380.x
Subject(s) - nucleolus , biology , transcription (linguistics) , rna , rna polymerase i , ribosomal rna , rna polymerase , microbiology and biotechnology , cell , gene , genetics , nucleus , linguistics , philosophy
Recent electron microscopic immunolocalization studies (Scheer and Rose, 1984) indicated that RNA polymerase I is located in, and probably confined to, the fibrillar centres of the nucleoli. This conclusion appears to be in contradiction to previous autoradiographic studies reporting that transcription of rRNA genes takes place in the dense fibrillar component. Despite the fact that rRNA transcription occurs in the dense fibrillar component of Ehrlich tumour cell nucleoli, we show in the present paper that dense fibrillar component containing rapidly labelled RNA can be visualized not only around but also inside the fibrillar centres.