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Enhancement of glomerular permeability to anionic ferritin induced by kidney perfusion with collagenase
Author(s) -
Schaeverbeke J.,
Lalande H. Moreau,
GelosoMeyer A.,
Perichon M.,
BorotLaloi C.,
Cheig M.
Publication year - 1985
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1985.tb00365.x
Subject(s) - ferritin , collagenase , glomerulus , perfusion , biology , vascular permeability , basement membrane , biophysics , kidney , glomerular basement membrane , permeability (electromagnetism) , renal glomerulus , membrane permeability , membrane , enzyme , biochemistry , microbiology and biotechnology , medicine , endocrinology , glomerulonephritis
The role of collagen in ultrafiltration properties of the glomerular basement membrane (GBM) was tested after a single administration of bacterial collagenase, using native ferritin as a tracer which does not pass through the GBM under physiological conditions. Experiments were performed both in situ and with isolated kidneys. Increased permeability to ferritin occurs 6 hr following enzyme perfusion and becomes patent after 30 hr, numerous tracer molecules appearing in urinary space, without any readily observable changes either in distribution of fixed negative charges (as revealed by colloidal iron and polyethyleneimine) or in structural organization of the glomerulus. Selective permeability of the GBM is progressively restored so that ferritin is almost confined to capillary lumen one month after enzyme injection. We conclude that collagen plays an important part in restricting plasma protein filtration.