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Fluid phase and mannose receptor‐mediated uptake of horseradish peroxidase in mouse bone marrow‐derived macrophages. Biochemical and ultrastructural study
Author(s) -
Lang T.,
Chastellier C.
Publication year - 1985
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1985.tb00362.x
Subject(s) - horseradish peroxidase , biology , endocytosis , mannose , extracellular , mannose receptor , peroxidase , mannan , ultrastructure , receptor , in vitro , bone marrow , microbiology and biotechnology , biochemistry , phagocytosis , yeast , enzyme , macrophage , immunology , anatomy , polysaccharide
A detailed study of horseradish peroxidase (HRP) uptake by in vitro cultured bone marrow‐derived macrophages was undertaken. Biochemical quantitations performed over a wide range of HRP concentrations, in the absence or presence of yeast mannan, showed that these macrophages pinocytose HRP by both fluid phase and mannose receptor‐mediated uptake. The relative contribution of these two types of endocytosis varied with the concentration of enzyme in the extracellular medium. A morphological study at the light and electron microscope levels conducted in parallel confirmed the biochemical data.