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Effects of the vital DNA probe fluorochrome Hoechst 33342 on PtK cell chromatin
Author(s) -
Gregoire M.,
HernandezVerdun D.,
Bouteille M.
Publication year - 1985
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1985.tb00348.x
Subject(s) - chromatin , staining , biology , microbiology and biotechnology , transcription (linguistics) , dna , fragmentation (computing) , vital stain , cell , cell cycle , electron microscope , biochemistry , genetics , ecology , linguistics , philosophy , physics , optics
We investigated the effects of DNA labelling by fluorochrome Hoechst 33342, on chromatin distribution and transcription during a short‐term non‐toxic staining procedure which did not prevent the cell growth. By electron microscope examination we found chromatin condensation and nucleolar fragmentation just after the staining. Both effects appeared to be reversible 24h after staining; 48h afterwards, the morphological pattern was similar to that of control cells. The rate of RNA transcription was reduced at the end of staining, increased at 24h, and was comparable to the control at 48h. We also observed an effect on the cell cycle when the staining pasted 6h. Consequently, 24h after Hoechst vital staining, the effects on chromatin vanished whereas the cells remained fluorescent.