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Three‐dimensional reconstruction of innermost chorion layer from Drosophila melanogaster
Author(s) -
Margaritis L. H.,
Hamodrakas S. J.,
Arad T.,
Leonard K. R.
Publication year - 1985
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1985.tb00347.x
Subject(s) - biology , layer (electronics) , electron microscope , drosophila melanogaster , resolution (logic) , drosophila (subgenus) , biophysics , crystallography , space (punctuation) , anatomy , optics , materials science , physics , nanotechnology , biochemistry , chemistry , computer science , artificial intelligence , gene , operating system
A low‐resolution three‐dimensional structure of the crystalline innermost chorion layer (ICL) has been calculated from electron microscope images of tilted negatively stained crystals. The isolated ICL is a single layer, about 12 nm thick and appears to be made up of two types of subunits, each approximately 3 nm in diameter, arranged regularly as groups of four heterodimers in space group C222. Linking density between these groups of subunits, maintaining the integrity of the layer, appears to be confined mainly to the outer surfaces of the ICL.