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Distribution of U3 snRNA in the nucleolus of CHO cells
Author(s) -
LacosteRoyal G.,
Simard R.
Publication year - 1985
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1985.tb00335.x
Subject(s) - nucleolus , biology , ribonucleoprotein , small nuclear rna , chromatin , rna , microbiology and biotechnology , ribosomal rna , ultrastructure , biophysics , nucleus , biochemistry , anatomy , dna , rna dependent rna polymerase , gene
Localization of snRNA at the ultrastructural level was studied in the nucleolus of CHO cells by EM autoradiography. In conditions where snRNA U3 is the only RNA species labelled in the nucleolus, silver grains were largely found at the periphery, over the granular ribonucleoprotein component and the perinucleolar condensed chromatin; this enrichment was quantitatively significant. Inhibition of pre‐rRNA synthesis with actinomycin D did not alter the concentration or the distribution of U3 inside the nucleolus. The results are consistent with the demonstration that U3 is hydrogen‐bonded to 28S pre‐rRNA, and thus should be found in the granular compartment where 32S‐28S pre‐rRNA is assembled into 55s RNP.