Premium
Pushing the signal hypothesis: what are the limits?
Author(s) -
Hortsch M.,
Meyer D. I.
Publication year - 1985
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1985.tb00319.x
Subject(s) - endoplasmic reticulum , biology , microbiology and biotechnology , signal peptide , membrane , translation (biology) , biophysics , translocon , membrane protein , computational biology , biochemistry , peptide sequence , messenger rna , gene
Recent advances in understanding the in vitro translocation of nascent polypeptides across the endoplasmic reticulum (ER) membrane have established a molecular basis for the initial reactions predicted by the signal hypothesis. The first two events involve a transient arrest of nascent chain elongation, followed by a docking maneuver with the ER membrane which releases this block. It is not clear, however, that such signal sequence‐mediated transfer occurs in the case of all proteins, or for that matter in all cell‐free translation systems.