Premium
Culture of chondrocytes in medium supplemented with fetal calf serum or a serum substitute: Ultroser G
Author(s) -
Ronot X.,
Sene C.,
Boschetti E.,
Hartmann D. J.,
Adolphe M.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00309.x
Subject(s) - biology , chondrocyte , fetal bovine serum , fetus , andrology , cartilage , cell growth , endocrinology , medicine , type ii collagen , cell culture , immunology , cell , anatomy , biochemistry , pregnancy , genetics
Fetal calf serum and a serum substitute, Ultroser G, were compared for their effects on the growth curves, clonal growth and cell cycle progression of rabbit chondrocytes in primary culture and during at least three cell passages and included a screen for the maintenance of cartilage‐like differentiation i.e. the presence of type II collagen. Proliferation was also compared with another serum substitute, Nu‐Serum. Ultroser G is shown to be equivalent to fetal calf serum as far as chondrocyte proliferation is concerned, clonal growth is improved and biosynthesis of type II collagen is maintained in primary culture.