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Semliki Forest virus penetration from endosomes: a morphological study
Author(s) -
Helenius A.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00297.x
Subject(s) - semliki forest virus , endosome , biology , virus , ammonium chloride , lipid bilayer fusion , penetration (warfare) , microbiology and biotechnology , virology , biophysics , biochemistry , intracellular , chemistry , rna , organic chemistry , gene , operations research , engineering
The low pH dependent membrane fusion reaction responsible for the delivery of the Semliki Forest virus genome into the host cell for replication was visualized by electron microscopy. In order to increase the frequency at which fusion images could be detected a reversible inhibitor, ammonium chloride, was used to synchronize endosomal acidification, and 20 degrees C incubation was employed to concentrate virus particles into the endosomal compartment.