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Colocalization of U1 and U2 small nuclear RNPs by immunocytochemistry
Author(s) -
Spector D. L.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00289.x
Subject(s) - snrnp , colocalization , biology , immunoelectron microscopy , cajal body , interphase , microbiology and biotechnology , immunocytochemistry , cell nucleus , ribonucleoprotein , immunofluorescence , nucleus , rna , rna splicing , immunohistochemistry , genetics , immunology , gene , antibody , endocrinology
The in situ localization of U1 and U2 snRNPs was examined, using autoantibodies directed against each of these snRNPs, by immunofluorescence and immunoelectron microscopy. This study has shown U1 and U2 snRNPs to colocalize in the nuclei of PtK2 cells. Thirty to fifty immunostained clusters were observed per interphase nucleus. This study suggests these nuclear protein clusters to be the sites of RNA processing.