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Human B‐like lymphoblastoid cell lines obtained by long‐term culture of normal spleen leukocytes
Author(s) -
Lombard Y.,
Hartmann D.,
Coupin G.,
Benezra R.,
Poindron P.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00276.x
Subject(s) - biology , sendai virus , lymphoblast , spleen , cell culture , antibody , virology , microbiology and biotechnology , virus , antigen , ploidy , cell , immunology , biochemistry , gene , genetics
B‐like lymphoblastoid cell lines were obtained by long‐term culture of human spleen leukocytes in RPMI 1640 medium containing human plasma fraction instead of whole foetal calf serum. These cell lines, which did not form E‐rosettes had membrane immunoglobulins, and expressed Epstein‐Barr virus antigens. Most synthesized intracytoplasmic immunoglobulins and were shown to be diploid and to remain so after subcultures. All produced interferon upon induction with Sendai virus.