Premium
RNA synthesis in pig follicular oocytes. Autoradiographic and cytochemical study
Author(s) -
Motlík J.,
Kopecný V.,
Trávnik P.,
Pivko J.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00270.x
Subject(s) - nucleolus , nucleoplasm , biology , rna , oogenesis , labelling , microbiology and biotechnology , silver stain , oocyte , staining , follicular phase , andrology , nucleus , embryo , biochemistry , genetics , gene , medicine
RNA synthesis in pig oocytes was studied using autoradiography and silver staining of the nucleolus organizing region. Both methods confirmed that oocytes from the smallest follicles (0.5‐0.7 mm in diam.) very intensely synthesize nuclear and nucleolar RNA. The nucleolar area of oocytes originating from follicles of 1.6‐2.2 mm in diam. was labelled mainly on its periphery. After short pulse labelling (15 min) of oocytes from follicles of 5‐6 mm in diam. only the nucleoplasm was labelled. The nucleolus had no significant labelling. The possibility that labelling of the compact nucleolus after a longer pulse represents migration of the newly synthesized nuclear RNA into the compact nucleolus, is discussed. The quantity of silver‐positive material in dictyate oocytes significantly decreased as pig follicles enlarged in diam. from 2 mm to 5‐6 mm.