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Improved coating and fixation methods for scanning electron microscope autoradiography
Author(s) -
Weiss R. L.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00262.x
Subject(s) - scanning electron microscope , coating , fixation (population genetics) , electron microscope , emulsion , materials science , microscope , photographic emulsion , optics , biology , biomedical engineering , composite material , physics , medicine , biochemistry , gene , silver halide
A simple apparatus for emulsion coating is described. The apparatus is inexpensive and easily assembled in a standard glass shop. Emulsion coating for scanning electron microscope autoradiography with this apparatus consistently yields uniform layers. When used in conjunction with newly described fixation methods, this new approach produces reliable autoradiographs of undamaged specimens.