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Interactions between human fibroblasts and HeLa cells in vitro
Author(s) -
Delinassios J. G.,
Kottaridis S. D.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00250.x
Subject(s) - hela , biology , stromal cell , fibroblast , in vitro , cell culture , thymidine , heterologous , cell , microbiology and biotechnology , dna , tissue culture , biochemistry , cancer research , genetics , gene
Affinity toward each other was demonstrated in co‐cultures between HeLa cells and fibroblasts originating from human tumor stromal or normal tissues. Both cell types in the mixed cultures (ratio 1:1, 1:2, 2:1) proliferated normally as shown by 3H‐thymidine labeling index estimation for up to 48 hr of co‐culture. At ratios of fibroblasts: HeLa lower than 1:10, fibroblasts were eventually eliminated after serial passaging. It was shown that 3H‐nucleotides could be transferred between heterologous cells in either direction. Contact of cells was essential for this phenomenon. Transfer of the label from HeLa to fibroblasts required a longer interaction time and was evidently lower than the transfer from fibroblasts to HeLa. 3H‐thymidine incorporated into the DNA of either cell type could not be transferred from one cell to another. The model provides a means for studying neoplastic X normal (or tumour stromal) cell interactions in vitro.