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Ultrastructural aspects after cryoultramicrotomy of bone tissue and sutural cartilage in neonatal mice calvaria
Author(s) -
Boivin G.,
Morel G.,
Meunier P. J.,
Dubois P. M.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00241.x
Subject(s) - calvaria , ultrastructure , biology , cartilage , anatomy , matrix (chemical analysis) , extracellular matrix , microbiology and biotechnology , vesicle , bone cell , pathology , membrane , chemistry , biochemistry , medicine , chromatography , in vitro
Using transmission electron microscopy after cryoultramicrotomy, mineralized as well as unmineralized bone tissues and sutural cartilage were observed in neonatal mice calvaria. A good definition of osteoblasts (nucleus, membranes, organelles) and extracellular constituents (collagen fibrils, matrix vesicles, mineral substance) was obtained. The sutural zone was composed of an unmineralized cartilaginous tissue with more or less hypertrophic cells surrounded by a finely fibrillar matrix.