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Basement membrane maintenance in cultures of isolated thyroid follicles
Author(s) -
Alquier C.,
AthouelHaon A. M.,
Mazzella E.,
MichelBechet M.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00235.x
Subject(s) - collagenase , basement membrane , biology , fibronectin , thyroid , in vitro , medicine , endocrinology , hormone , microbiology and biotechnology , biochemistry , extracellular matrix , enzyme
Follicles from adult rat thyroid glands were isolated by digestion with collagenase followed by sieving. Electron microscopic studies have shown that basement membrane was present around each one of the isolated follicles. Follicles were cultured in vitro with or without thyrotropin and fibronectin. When cultured in a medium free of thyrotropin, basement membrane remained around them, while it was lacking in presence of this hormone.