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Ultrastructural localization of DNA in tissue culture cell nuclei by means of enzyme‐gold and autoimmune sera‐protein A‐gold techniques
Author(s) -
Raska I.,
Armbruster B. L.,
Jíra M.,
Hána I.,
Kaslík J.,
Rovenský J.,
Smetana K.
Publication year - 1984
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1984.tb00214.x
Subject(s) - nucleolus , euchromatin , biology , chromatin , dna , microbiology and biotechnology , cell nucleus , ultrastructure , cell , biophysics , cytoplasm , biochemistry , heterochromatin , anatomy
The post‐embedding localization of DNA was investigated in cell nuclei by means of DNase I‐gold and autoimmune sera‐protein A‐gold techniques. Using the former technique, gold particles were found mainly over euchromatin, nucleoli exhibit occasionally high labeling. In the latter technique, the use of the serum binding dsDNA confined the label mainly to condensed chromatin.