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The P seudomonas aeruginosa antimetabolite L ‐2‐amino‐4‐methoxy‐ trans ‐3‐butenoic acid inhibits growth of E rwinia amylovora and acts as a seed germination‐arrest factor
Author(s) -
Lee Xiaoyun,
Azevedo Mark D.,
Armstrong Donald J.,
Banowetz Gary M.,
Reimmann Cornelia
Publication year - 2013
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/j.1758-2229.2012.00395.x
Subject(s) - biology , microbiology and biotechnology
Summary The P seudomonas aeruginosa antimetabolite L ‐2‐amino‐4‐methoxy‐ trans ‐3‐butenoic acid ( AMB ) shares biological activities with 4‐formylaminooxyvinylglycine, a related molecule produced by P seudomonas fluorescens WH 6. We found that culture filtrates of a P . aeruginosa strain overproducing AMB weakly interfered with seed germination of the grassy weed P oa annua and strongly inhibited growth of E rwinia amylovora , the causal agent of the devastating orchard crop disease known as fire blight. AMB was active against a 4‐formylaminooxyvinylglycine‐resistant isolate of E . amylovora , suggesting that the molecular targets of the two oxyvinylglycines in E rwinia do not, or not entirely, overlap. The AMB biosynthesis and transport genes were shown to be organized in two separate transcriptional units, ambA and ambBCDE , which were successfully expressed from IPTG ‐inducible tac promoters in the heterologous host P . fluorescens CHA 0. Engineered AMB production enabled this model biocontrol strain to become inhibitory against E . amylovora and to weakly interfere with the germination of several graminaceous seeds. We conclude that AMB production requires no additional genes besides ambABCDE and we speculate that their expression in marketed fire blight biocontrol strains could potentially contribute to disease control.