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Human E scherichia coli strains of different geographical and time source: bacteriocin types and their gene sequences are population‐specific
Author(s) -
Šmajs David,
Čejková Darina,
Micenková Lenka,
LimaBittencourt Claudia I.,
ChartoneSouza Edmar,
Šmarda Jan,
Nascimento Andréa M. A.
Publication year - 2012
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/j.1758-2229.2012.00365.x
Subject(s) - bacteriocin , colicin , biology , gene , escherichia coli , genetics , microbiology and biotechnology , population , bacteria , demography , sociology
Summary Bacteriocin production was tested in two sets of E scherichia coli strains: one isolated in 1978 from healthy children of rubber tree tappers and Indians in Amazonia, B razil ( n = 35), and the second one isolated in 2004 from healthy adult volunteers in the M oravia region, C zech R epublic ( n = 53). Although the occurrence of bacteriocin production was rather similar in both groups (54.3% and 43.4% respectively), the spectra of bacteriocin‐encoding determinants in both groups were different. Altogether, 12 different bacteriocin‐encoding determinants were found among the tested strains. The occurrence of colicin E 1‐ and Y ‐genes was higher ( P = 0.02 and P = 0.009 respectively) while the occurrence of microcin V gene was lower ( P = 0.02) among A mazonian strains compared with M oravian strains. The colicin‐encoding determinants of colicins Ia, M , Y and E 1 were amplified from A mazonian and M oravian producer strains and sequenced, as were chromosomal 16S rRNA , gyr B and ton B genes. While sequence alignments of chromosomal loci revealed no clusters with respect to geographical origin of strains, the colicin‐encoding genes were very similar among the strains of each origin but different between the two geographic groups.