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Structural alterations of faecal and mucosa‐associated bacterial communities in irritable bowel syndrome
Author(s) -
Durbán Ana,
Abellán Juan J.,
JiménezHernández Nuria,
Salgado Patricia,
Ponce Marta,
Ponce Julio,
Garrigues Vicente,
Latorre Amparo,
Moya Andrés
Publication year - 2012
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/j.1758-2229.2012.00327.x
Subject(s) - irritable bowel syndrome , feces , gastroenterology , biology , 16s ribosomal rna , microbiome , medicine , bacteria , microbiology and biotechnology , bioinformatics , genetics
Summary Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder in western countries. Previous studies on IBS, mostly based on faecal samples, suggest alterations in the intestinal microbiota. However, no consensus has been reached regarding the association between specific bacteria and IBS. We explore the alterations of intestinal bacterial communities in IBS using massive sequencing of amplified 16S rRNA genes. Mucosal biopsies of the ascending and descending colon and faeces from 16 IBS patients and 9 healthy controls were analysed. Strong inter‐individual variation was observed in the composition of the bacterial communities in both patients and controls. These communities showed less diversity in IBS cases. There were larger differences in the microbiota composition between biopsies and faeces than between patients and controls. We found a few over‐represented and under‐represented taxa in IBS cases with respect to controls. The detected alterations varied by site, with no changes being consistent across sample types.