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Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities
Author(s) -
Knierim Bernhard,
Luef Birgit,
Wilmes Paul,
Webb Richard I.,
Auer Manfred,
Comolli Luis R.,
Banfield Jillian F.
Publication year - 2012
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/j.1758-2229.2011.00275.x
Subject(s) - correlative , ultrastructure , in situ , biology , phylogenetic tree , fish <actinopterygii> , fluorescence in situ hybridization , transmission electron microscopy , microscopy , fluorescence microscope , evolutionary biology , fluorescence , nanotechnology , chemistry , anatomy , materials science , fishery , pathology , gene , genetics , optics , medicine , philosophy , linguistics , physics , organic chemistry , chromosome
Summary Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.