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Involvement of poly(3‐hydroxybutyrate) synthesis in catabolite repression of tetralin biodegradation genes in Sphingomonas macrogolitabida strain TFA
Author(s) -
MartínCabello Guadalupe,
MorenoRuiz Emilia,
Morales Valle,
Floriano Belén,
Santero Eduardo
Publication year - 2011
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/j.1758-2229.2011.00273.x
Subject(s) - catabolite repression , strain (injury) , biodegradation , sphingomonas , tetralin , microbiology and biotechnology , psychological repression , gene , chemistry , bacterial strain , biology , bacteria , biochemistry , genetics , organic chemistry , 16s ribosomal rna , gene expression , mutant , anatomy , catalysis
Summary The tetralin biodegradation genes of Sphingomonas macrogolitabida strain TFA are repressed by catabolite. Insertion mutants in which thn genes are transcribed in the presence of a preferential carbon source and tetralin, bear the insertion in phaC , encoding a poly(3‐hydroxybutyrate) (PHB) synthase, a key enzyme in PHB synthesis. Mutant complementation with phaC genes from either Ralstonia euthropha or TFA restored PHB accumulation and the wild‐type regulatory pattern of thn genes, thus indicating that this accumulation is a signal for carbon sufficient conditions that prevents expression of thn catabolic genes in this α‐proteobacteria.