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Antibiotic selection leads to inadvertent selection of nfxC ‐type phenotypic mutants in Pseudomonas aeruginosa
Author(s) -
Mooij Marlies J.,
O'Connor Hazel F.,
Tian ZheXian,
Wang YiPing,
Adams Claire,
Morrissey John P.,
O'Gara Fergal
Publication year - 2010
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/j.1758-2229.2010.00175.x
Subject(s) - pseudomonas aeruginosa , mutant , phenotype , biology , efflux , microbiology and biotechnology , wild type , genetics , bacteria , gene
Summary Spontaneous nfxC ‐type phenotypic mutants of Pseudomonas aeruginosa are characterized by chloramphenicol resistance and increased expression of the multidrug efflux pump, MexEF‐OprN. These mutants frequently arise during the propagation of bacteria in laboratory conditions. This study shows that some of the discrepancies in transcriptomic/phenotypic profiles of two rsmA mutants, PAZH13 ( rsmA mutant in strain PAO1) and PAKΔ rsmA , are due to the nfxC ‐type nature of PAZH13. This indicates that awareness of the possible occurrence of these spontaneous nfxC ‐type phenotypes is necessary. Screening of mexE levels could provide clarity when an nfxC ‐type phenotype is suspected.