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Differences in stress response after UVC or UVA irradiation in Vibrio parahaemolyticus
Author(s) -
Hamamoto Akiko,
Bandou Chiyo,
Nakano Masayuki,
Mawatari Kazuaki,
Lian Xin,
Yamato Masayuki,
Harada Nagakatsu,
Akutagawa Masatake,
Kinouchi Yohsuke,
Nakaya Yutaka,
Takahashi Akira
Publication year - 2010
Publication title -
environmental microbiology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.229
H-Index - 69
ISSN - 1758-2229
DOI - 10.1111/j.1758-2229.2010.00154.x
Subject(s) - repressor lexa , sos response , vibrio parahaemolyticus , biology , mutant , dna damage , escherichia coli , filamentation , dna repair , wild type , gene , microbiology and biotechnology , dna , genetics , gene expression , bacteria , repressor , laser , physics , optics
Summary The SOS response is a global regulatory network for repairing DNA damage induced by various environmental stresses such as UV irradiation. The Escherichia coli SOS response has been extensively studied. However, there are no reports on the SOS response in Vibrio parahaemolyticus . In this study, we examined the SOS response in V. parahaemolyticus and compared the differential expression of genes induced by UVC and UVA irradiation. In UVC‐exposed wild‐type cells, expression of several DNA repair genes was increased. However, expression of these genes was not increased in Δ recA or lexA mutants. Cell filamentation was observed in wild‐type cells, but not in Δ recA and lexA mutant cells. Sensitivity to UVC was significantly increased in Δ recA , lexA mutant and Δ lon strains compared with wild type. In the case of UVA irradiation, LexA‐controlled DNA repair genes were minimally induced and cell filamentation was not observed. Sensitivity to UVA was the same in the mutant and wild‐type strains. These findings suggest that there is a RecA‐LexA‐mediated SOS response in V. parahaemolyticus , and that this response is important to UVC tolerance but does not contribute to UVA tolerance.