Open AccessEctopic osteogenesis of mouse bone marrow stromal cells transfected with BMP 2/VEGF 165 genes in vivo
Author(s) -
Tian Xiaobin,
Sun Li,
Yang Shuhua,
Fu Ruyin,
Wang Lei,
Lu Tinshen,
Zhang Yukun,
Fu Dehao
Publication year - 2009
Publication title -
orthopaedic surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 23
eISSN - 1757-7861
pISSN - 1757-7853
DOI - 10.1111/j.1757-7861.2009.00045.x
Subject(s) - bone morphogenetic protein 2 , stromal cell , transfection , bone marrow , vascular endothelial growth factor , neovascularization , angiogenesis , pathology , microbiology and biotechnology , biology , cancer research , medicine , cell culture , vegf receptors , in vitro , biochemistry , genetics
Objective: To evaluate the osteogenic efficacy of mouse bone marrow stromal cells (mBMSC) transfected with bone morphogenetic protein 2 (BMP2) and vascular endothelial growth factor 165 (VEGF 165 ) genes. Methods: pIRES‐BMP2‐VEGF 165 plasmid DNA was introduced into the mBMSC using a liposome‐mediated method. The expression of BMP2 and VEGF 165 genes was assessed by reverse transcription‐polymerase chain reaction (RT‐PCR) and immunohistochemical analysis. Transfected cells were injected into the thigh muscle pouches of four nude mice. The osteoinductivity activity of the transfected cells was evaluated by radiographic and histological analysis at 4 weeks after injection. Results: The mRNA and proteins of both BMP2 and VEGF 165 were successfully expressed in mBMSC as confirmed by RT‐PCR and immunohistochemical analysis. Ectopically formed bone tissue was clearly observed at 4 weeks after cell injection in the thigh muscle pouches of the nude mice. Conclusion: mBMSC transfected with BMP2 and VEGF 165 genes can induce ectopic osteogenesis.