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Urothelial α 1D Receptor is Predominantly Involved in the Adrenergic Facilitation of Micturition Reflex
Author(s) -
WANG Xiaojun,
MATSUMOTOMIYAI Kazumasa,
YOSHIZUMI Masaru,
ITO Tomoko,
YANASE Haruko,
MOMOTA Yoshiharu,
TSUJIMOTO Gozoh,
TANOUE Akito,
NIMURA Toshie,
KAWATANI Masahito
Publication year - 2009
Publication title -
luts: lower urinary tract symptoms
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.451
H-Index - 15
eISSN - 1757-5672
pISSN - 1757-5664
DOI - 10.1111/j.1757-5672.2009.00019.x
Subject(s) - urothelium , urination , cystometry , reflex , urinary bladder , endocrinology , medicine , receptor , urinary system , urology
Previous studies using specific antagonists have shown that α 1 ‐adrenergic receptors (AR) in the urinary bladder epithelium (urothelium) serve as a regulator of micturition reflex. To confirm the urothelial adrenergic effect on the micturition reflex, we investigated the functional role of the α 1D ‐AR subtype in the urothelium on micturition reflex using α 1D ‐AR knockout (α 1D ‐KO) mice. An immunohistochemical analysis showed that the α 1D ‐AR was localized to the umbrella cells in the mouse urothelium. Cystometry using wild‐type mice demonstrated that the intravesical infusion of noradrenaline (NA) into the urinary bladder shortened the intercontraction interval (ICI) in a dose‐dependent manner, without changing the maximum voiding pressure (MVP). In contrast, α 1D ‐KO mice exhibited no change in the ICI in response to the intravesical infusion of NA. These results indicate a predominant involvement of the α 1D ‐AR in facilitation of micturition reflex by NA. Interestingly, the intravenous injection of NA did not alter the ICI and the MVP in wild‐type mice. Thus, locally releasing NA might play an important role in the facilitation of micturition reflex via the urothelial α 1D−AR subtype.