Open AccessDirect shoot organogenesis from hypocotyl explants of J atropha curcas L . an important bioenergy feedstock
Author(s) -
Sahoo Nirmala,
Thirunavoukkarasu Manikannan,
Behera Priya Ranjan,
Satpathy Gargi Bala,
Panda Prasanna Kumar
Publication year - 2012
Publication title -
gcb bioenergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.378
H-Index - 63
eISSN - 1757-1707
pISSN - 1757-1693
DOI - 10.1111/j.1757-1707.2011.01120.x
Subject(s) - explant culture , shoot , kinetin , hypocotyl , murashige and skoog medium , organogenesis , botany , biology , micropropagation , axillary bud , horticulture , chemistry , in vitro , biochemistry , gene
Multiple shoots were induced on hypocotyl segments reared from in vitro germinated seedlings of J atropha curcas L ., using M urashige and S koog ( MS ) medium supplemented with N 6 ‐benzyl adenine ( BA ) and kinetin (kin) either alone or in combination with indole‐3‐acetic acid ( IAA ). The combined treatment of 7.05 μ m kin and 1.425 μ m IAA resulted in maximum shoot production with an average shoot bud initiation of 12.1 per explant. The regenerated microshoots were transferred to root induction medium containing half‐strength MS salts supplemented with either indole‐3‐butyric acid ( IBA ) or IAA . Rooting of microshoots was best achieved on half‐strength MS medium supplemented with IBA (9.8 μ m ). Rooted plantlets were subsequently acclimatized under green house condition and the plantlets showed 70% survival.