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Radiographic findings of Wistar rats fed with T‐2 toxin and Kashin‐Beck disease‐affected diet
Author(s) -
YAN Denglu,
KANG Pengde,
LI Yansheng,
YANG Jing,
SHEN Bing,
ZHOU Zongke,
DENG Jianyun,
PEI Fuxing
Publication year - 2011
Publication title -
international journal of rheumatic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 41
eISSN - 1756-185X
pISSN - 1756-1841
DOI - 10.1111/j.1756-185x.2010.01568.x
Subject(s) - medicine , histopathology , tibia , h&e stain , toxin , epiphyseal plate , endocrinology , pathology , surgery , immunohistochemistry , biology , biochemistry
Objective: To characterize the features of radiographic abnormalities of the tibial bone in Wistar rats which have been fed T‐2 toxin and Kashin‐Beck disease (KBD) epidemic district food. Methods: Ninety Wistar rats were divided into five groups. Group A was fed with a normal diet as control; group B was fed with a normal diet and T‐2 toxin; group C was fed with a low‐nutrition diet and T‐2 toxin; group D was fed with a low‐nutrition diet; and group E was fed with a KBD‐affected diet. At 4, 8 and 12 weeks, six rats from each group were radiographed. After radiographic examination, samples of left knees were harvested and stained with hematoxylin and eosin. Results: At 8 and 12 weeks, there were radiological changes in the epiphyseal plate. Abnormal radiological signs of blurring, thinning and irregularity were seen in groups C and E rats, and the length of tibial bones showed significant difference in the KBD‐fed rats and low‐nutrition diet combined T‐2 toxin rats, compared to the control group rats ( P < 0.05). The epiphyseal plates showed more obvious necrosis of chondrocytes in groups C and E. Conclusions: A rat model of KBD can be established by a KBD‐affected diet; proximal epiphyseal plate and metaphyseal bone of the tibia abnormalities on radiographs and histopathology were present in KBD model rats. A low nutrition diet may be involved the aetiology of KBD, and determination of this should be studied in the future.