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The relationship between single nucleotide polymorphisms of the interleukin‐10 gene promoter in systemic lupus erythematosus patients in Malaysia: a pilot study
Author(s) -
HEE Chee Seng,
GUN Suk Chyn,
NAIDU Rakesh,
SOMNATH Sushela Devi,
RADHAKRISHNAN Ammu Kutty
Publication year - 2008
Publication title -
international journal of rheumatic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 41
eISSN - 1756-185X
pISSN - 1756-1841
DOI - 10.1111/j.1756-185x.2008.00350.x
Subject(s) - single nucleotide polymorphism , haplotype , genotype , pathogenesis , restriction fragment length polymorphism , promoter , immunology , allele frequency , allele , medicine , interleukin , genotype frequency , gene , polymerase chain reaction , polymorphism (computer science) , genetics , biology , gene expression , cytokine
Aim: Recent studies have shown that single nucleotide polymorphisms (SNPs) have been identified within the promoter of the human interleukin‐10 (IL‐10) gene may participate in the pathogenesis of systemic lupus erythematosus (SLE) and may be related to disease activity. This is a pilot study that investigated the allelic and genotype frequencies of three SNPs in the human IL‐10 gene promoter [rs1800896 (position: –1082G > A), rs1800871 (position: –824C > T) and rs1800872 (position: –597C > A)] among Malaysian SLE patients and normal subjects. Methods: Blood was drawn from 44 SLE patients and 44 age‐ and sex‐matched healthy control subjects for DNA extraction. The SNPs were identified using the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) method. Results: There was no significant difference in the genotype and allele frequencies between the SLE patients and control subjects. A statistically significant difference was detected in the haplotype frequencies between the patients and controls ( P = 0.004). Conclusions: There is a significant difference in the haplotype frequencies between the SLE patients and controls; the SNPs in the human IL‐10 gene promoter could play an important role in the pathogenesis of SLE.