Open AccessTransplantation of Adipose‐Derived Stem Cells is Associated with Neural Differentiation and Functional Improvement in a Rat Model of Intracerebral Hemorrhage
Author(s) -
Chen Juan,
Tang YingXin,
Liu YongMing,
Chen Ji,
Hu XiaoQing,
Liu Na,
Wang ShuXin,
Zhang Yu,
Zeng WenGao,
Ni HouJie,
Zhao Bin,
Chen YanFang,
Tang ZhouPing
Publication year - 2012
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/j.1755-5949.2012.00382.x
Subject(s) - adipose tissue , transplantation , neural stem cell , stem cell , intracerebral hemorrhage , astrocyte , pathology , medicine , biology , microbiology and biotechnology , central nervous system , subarachnoid hemorrhage
Summary Aims To examine whether transplantation of adipose‐derived stem cells ( ADSC s) induces neural differentiation and improves neural function in a rat intracerebral hemorrhage ( ICH ) model. Methods Adipose‐derived stem cells cells were isolated from inguinal fat pad of rat. ICH was induced by injection of collagenase type IV into the right basal ganglia of rat. Forty‐eight hours after ICH , ADSC s cells (10 μL of 2–4 × 10 7 cells/ mL ) were injected into the right lateral cerebral ventricle. The differentiation of ADSC s was detected in vitro and in vivo . The neural function was evaluated with Z ea L onga 5‐grade scale at day 1, 3, 7, 14, or 28. Results Our data demonstrated that ADSC s differentiated into cells that shared the similarities of neurons or astrocytes in vitro . Transplantation of ADSC s decreased cell apoptosis and the transplanted ADSC s were able to differentiate into neuron‐like and astrocyte‐like cells around the hematoma, accompanied with upregulation of vascular endothelial growth factor expression and improvement of neural function. Conclusions Our data suggest that transplantation of ADSC s could be a therapeutic approach for ICH stroke.