Open AccessKallikrein Gene Transfer Induces Angiogenesis and Further Improves Regional Cerebral Blood Flow in the Early Period After Cerebral Ischemia/Reperfusion in Rats
Author(s) -
Lu RuiYan,
Luo DanFeng,
Xiao SongHua,
Yang LianHong,
Zhao Jia,
Ji ErNi,
Tao EnXiang,
Xing YiGang,
Zhu FengYing,
Luan Ping,
Liu Jun
Publication year - 2012
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/j.1755-5949.2012.00305.x
Subject(s) - cerebral blood flow , angiogenesis , ischemia , medicine , kallikrein , period (music) , cardiology , anesthesia , biology , biochemistry , enzyme , physics , acoustics
SUMMARY Aims: The aims of this study were to find out whether kallikrein could induce angiogenesis and affect the cerebral blood flow (rCBF) in the early period after cerebral ischemia/reperfusion (CI/R). Methods: The adenovirus carried human tissue kallikrein (HTK) gene was administrated into the periinfarction region after CI/R. At 12, 24, and 72 h after treatments, neurological deficits were evaluated; expression of HTK and vascular endothelial growth factor (VEGF) were detected by immunohistochemistry staining; the infarction volume was measured; and rCBF was examined by 14 C‐iodoantipyrine microtracing technique. Results: The expression of VEGF was enhanced significantly in pAdCMV‐HTK group than controls over all time points ( P < 0.05). Furthermore, the rCBF in pAdCMV‐HTK group increased markedly than controls at 24 and 72 h after treatment ( P < 0.05), and the improved neurological deficit was accompanied by reduced infarction volume in pAdCMV‐HTK group 24 and 72 h posttreatment. Conclusion: In the early period after CI/R, kallikrein could induce the angiogenesis and improve rCBF in periinfarction region, and further reduce the infarction volume and improve the neurological deficits.