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Difference of corneal endothelial cells according to senescence
Author(s) -
Joo Shin Young,
Sun Hwang Jin,
Kyung Jang Yoon
Publication year - 2019
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2019.5057
Subject(s) - viability assay , oxidative stress , cornea , cell , bullous keratopathy , cell cycle , cell growth , senescence , biology , mitochondrion , endothelial stem cell , andrology , transplantation , microbiology and biotechnology , pathology , ophthalmology , medicine , in vitro , endocrinology , biochemistry
Purpose Bullous keratopathy is a painful corneal endothelial disease and its treatment requires corneal transplantation because human corneal endothelial cells (HCECs) do not proliferate in vivo. In this study, we evaluated differences between HCECs depending on senescence. Methods HCECs were cultured and were classified into two groups: cells with small size and mosaic pattern (normal group), and cells with large size and no mosaic pattern (SA group). Cell shape, cell viability, and cell proliferation rate were evaluated. Cell cycle analysis, mitochondrial oxidative stress, and mitochondrial membrane potential were assessed. Western blotting was performed to evaluate expression of various proteins. Results Cell shape and size were different between normal and SA groups. Cell viability and cell proliferation rates were found to be lower in the SA group. Cell cycle analysis, mitochondrial oxidative stress, and mitochondrial membrane potential revealed differences between normal and SA groups. Protein expression was different between the normal group and the SA group. Conclusions Differences in phenotype between normal and SA groups might contribute to the pathogenesis of corneal endothelial diseases. References Siu GD, Young AL and Jhanji V. Alternatives to corneal transplantation for the management of bullous keratopathy. Curr Opin Ophthalmol. 2014; 25(4):347–352. Choi SO, Jeon HS, Hyon JY, Oh YJ, Wee WR, Chung TY, Shin YJ and Kim JW. Recovery of Corneal Endothelial Cells from Periphery after Injury. PLoS One. 2015; 10(9):e0138076. Eghrari AO, Riazuddin SA and Gottsch JD. Overview of the Cornea: Structure, Function, and Development. Prog Mol Biol Transl Sci. 2015; 134:7–23.